Identification of Immunogenic T and B-Cell Epitope Peptides of Rubella Virus E1 Glycoprotein towards the Development of Highly Specific Immunoassays and Vaccine

Introduction: The Rubella virus has a worldwide occurrence and congenital syndromes are widely recognized as an emerging infection in several parts of the world. Miscarriage, perinatal mortality, stillbirth can develop pregnancy during first trimester. most frequent techniques for laboratory diagnosis IgM IgG-based serological detection methods. Such viral bacterial pathogen emphasizes development fast diagnostic devices; there is need enhanced quicker methods.
 Materials Methods: Search peptide vaccine with specific T B-cell epitopes was identified through bioinformatics-based approaches. These were utilizing available E1 glycoprotein sequence databases. outer-membrane glycoprotein, target protein prediction best antigens.
 Results: Using BepiPred2 program, potential epitope PFCNTPHGQLEVQVPPDPGD high conservation among rubella maximum surface exposed residues identified. IEDB, NetMHCpan, NetCTL programs, T-cell RPVALPRAL Predicted found to have promiscuous class-I major histocompatibility complex binding affinity super types, antigenicity scores, proteasomal cleavage. three-dimensional modeled structures created using I-TASSER online server highlighting predicted T- B- cell epitopes.
 Conclusion: B could be used immunoglobulin assay candidate peptide.